Sclerostin binds and regulates the activity of cysteine-rich protein 61.

Publication Type:

Journal Article

Source:

Biochemical and biophysical research communications, Volume 392, Issue 1, p.36-40 (2010)

Keywords:

3T3 Cellsdigestive disease, digestive deseases Animalsdigestive disease, digestive deseases Bone Morphogenetic Proteinsdigestive disease, digestive deseases Cell Movementdigestive disease, digestive deseases Cell Proliferationdigestive disease, digestive deseases Cysteine-Rich Protein 61digestive disease, digestive deseases Endothelium, Vasculardigestive disease, digestive deseases Genetic Markersdigestive disease, digestive deseases Humansdigestive disease, digestive deseases Micedigestive disease, digestive deseases Osteoblastsdigestive disease, digestive deseases Two-Hybrid System Techniques

Abstract:

Sclerostin, a secreted glycoprotein, regulates osteoblast function. Using yeast two-hybrid and direct protein interaction analyses, we demonstrate that sclerostin binds the Wnt-modulating and Wnt-modulated, extracellular matrix protein, cysteine-rich protein 61 (Cyr61, CCN1), which regulates mesenchymal stem cell proliferation and differentiation, osteoblast and osteoclast function, and angiogenesis. Sclerostin was shown to inhibit Cyr61-mediated fibroblast attachment, and Cyr61 together with sclerostin increases vascular endothelial cell migration and increases osteoblast cell division. The data show that sclerostin binds to and influences the activity of Cyr61.