DNA Conjugated SWCNTs Enter Endothelial Cells via Rac1 Mediated Macropinocytosis.

Publication Type:

Journal Article

Source:

Nano letters, Volume 12, Issue 4, p.1826-30 (2012)

Abstract:

Several applications of single-walled carbon nanotubes (SWCNT) as nanovectors in biological systems have been reported, and several molecular pathways of cellular entry have been proposed. We employed transmission electron microscopy, confocal fluorescent microscopy, and UV-vis spectroscopic analysis to confirm the internalization of DNA-SWCNT in human umbilical vein endothelial cells. Additionally, by using pharmacological inhibitors as well as genetic approaches, we have found that SWCNT is endocytosed through Rac1- GTPase mediated macropinocytosis in normal endothelial cells.